Movies of the GroEL ATPase cycle

Additional material for the paper by Roseman et al (1996) Cell 87, 241-251

Description

These movies are short sequences of GroEL structures in complex with different nucleotides and GroES, created from surface-rendered images of cryo EM 3D reconstructions. They should be viewed as a repeating loop, and give the impression of domain movements occurring during the ATPase cycle. The letter codes are EL, GroEL; ELD, GroEL-ADP; ELT, GroEL-ATP; ELSD, GroEL-GroES-ADP; ELST, GroEL-GroES-ATP. They should be viewed in 256-color or full colour mode. Each movie is provided in both MPEG and QuickTime format.

Additional Data

1 MPEG
QuickTime		This sequence shows the GroEL movements seen directly from the side (i.e., perpendicular to the 7-fold axis of the oligomer).
2 MPEG
QuickTime		The molecule is tipped forward to allow a view into the cavity of the top ring. The domain rotation caused by nucleotide binding continues into the large movement accompanying GroES binding.
3 MPEG
QuickTime		The sequence of 4 states is shown tipped backwards, revealing a different pattern of movement in the lower ring. The upper ring (at the back of the images) performs a continuous twisting motion, but the lower ring opens and shuts with radial movements of the domains in addition to the twisting.
4 MPEG
QuickTime		The GroEL-GroES complexes are coloured according to the significance of differences between their ATP and ADP states. Red regions are those which change significantly, and no significant differences are seen in the blue regions.
5 MPEG
QuickTime		The GroEL-GroES complexes cut in half, to show the movements around the cis (GroES-bound) and trans binding cavities.

Note