Mutations in GroEL - Page 2

Residues 238 - 518

NOTE: Residue numbering includes N-terminal Met.

Mutation

Effect

Reference

E238ANo GroES binding; Decreased OTC folding (release & aggregation)

(1)

L259SNo GroES binding; No OTC binding; No growth of LG6 strain

(1)

V263SNo GroES binding; No OTC binding; No growth of LG6 strain

(1)

V264SDecreased ATPase; No GroES binding; No OTC binding
No growth of LG6 strain

(1)

N265ANo GroES binding; Decreased OTC folding (no release)
No growth of LG6 strain

(1)

K277EDecreased ATPase; Small colonies of LG6 strain

(1)

F281DDecreased ATPase; decreased OTC folding (release & aggregation)
No growth of LG6 strain

(1)

D283KNo effect detected

(1)

K286ENo effect detected

(1)

A293ENo effect detected

(1)

L309KDecreased OTC refolding (decreased release); No growth of LG6 strain

(1)

L314KDecreased OTC refolding (decreased release)

(1)

G337S/I349EDecreased OTC refolding (released & aggregation)
No growth of LG6 strain

(1)

E338KNo effect detected

(1)

Y360EDecreased ATPase; No growth of LG6 strain

(1)

D361KNo GroES binding; Decreased OTC refolding (decreased release)

(1)

R368ANo effect detected

(1)

K371ENo effect detected

(1)

V381SNo effect detected

(1)

A383ENo ATPase; No GroES binding; No OTC refolding (no release)
No growth of LG6 strain

(1)

K390A/K393ANo effect detected

(1)

H401FNo effect detected

(1)

A405ENo ATPase; No OTC refolding (no release); No growth of LG6 strain

(1)

A406ENo ATPase; No GroES binding; Decreased OTC binding
Decreased OTC refolding, No growth of LG6 strain

(1)

E409ANo effect detected

(1)

E461KDecreased OTC refolding (decreased release); No growth of LG6 strain

(1)

C518SDecreased ATPase; Decreased GroEL stability

(2)

C518SDecreased GroEL stability in presence of ATP or ADP

(3)

C518S/C137SThis double mutant reverses the effects of the C518S
mutant listed just above

(3)

This information is accurate to the best of my knowledge. However, you should check the references cited for a more complete understanding of these mutations and their effects.

References

  1. Fenton et al (1994) Nature371: 614-619.
  2. Luo & Horowitz (1994) J. Biol. Chem. 269: 32151-32154.
  3. Horovitz et al (1994) J. Mol. Biol. 238: 133-138.

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Last Updated April 11, 1996