Images of Glycolysis Intermediates Net reaction:
Glucose + 2 HPO42- + 2ADP2- + 2NAD+ 2 pyruvate + 2ATP4- + 2NADH + 2H3O+
As written, the net reaction corresponds to steps 1-10 of glycolysis. The NADH produced is always recycled to NAD+ either by oxidative phosphorylation (with O2) or by a fermentation pathway (without O2).

Left Frame:

Right Frame:

Energetics of Glycolysis   4.184 · kJ <-kcal Factor

Reaction Step

G°'
(kJ/mol)

G°'
(kcal/mol)

1. Hexokinase.

-16.736

-4.0

2. Phosphoglucose isomerase.

1.6736

+0.4

3. Phosphofructokinase.

-14.2256

-3.4

4. Aldolase.

23.8488

+5.7

5. Triose phosphate isomerase.

7.5312

+1.8

6. Glyceraldehyde 3-phosphate dehydrogenase.

6.276

+1.5

7. Phosphoglycerate kinase.

-18.828

-4.5

8. Phosphoglyceromutase.

4.6024

+1.1

9. Enolase.

1.6736

+0.4

10. Pyruvate kinase.

-31.38

-7.5

Fates for Pyruvate.

F1. Lactate dehydrogenase.

-25.104

-6.0

F2. Pyruvate decarboxylase.

?

?

F2. Alcohol dehydrogenase.

?

?

from Stryer 2nd Ed. Table 12-2.