MichaelisMenton (MM) Kinetics  Extended MM equation for interacting sites  


Rectangular hyperbolic plot (n = Hill coefficient)  Doublereciprocal plot  
"Ya" = V / V_{max} = [S]^{n} / ([S]^{n} + K_{m} ) where n = 1 for a MM enzyme  V_{max }/ V = 1 + K_{m} / [S]^{n} where n = 1 for a MM enzyme  
Hill plot  Scatchard plot  
V/( V_{max}  V ) = "Ya"/"Yd" = [S]^{n} / K_{m} where n = 1 for a MM enzyme and "Yd" = (V_{max}  V ) / V_{max} = K_{m} / ( [S]^{n} + K_{m} )  (V / V_{max}) / [S]^{n} = ( 1 / K_{m} ) * (1  V / V_{max}) where n = 1 for a MM enzyme  
Direct linear plot (n = 1)  
V_{max} = V + ( V / [S] ) * K_{m} = "Y" = b + a"X" where

Continue the kinetic analysis of enzymes by opening the following Excel spreadsheet
Graphical Analysis of Enzyme
Kinetics
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©  Duane W. Sears Revised: July 27, 1998 