Hill Analysis of Interactive Ligand Binding (lig)
In this exercise, the ligand binding interactions between the bivalent ligand binding sites of symmetrical dicarboxylic acid are examined using a Hill plot. When fully protonated ("saturated"), a dicarboxylic acid undergoes a two-step dissociation reaction with decreasing hydronium ion concentration. When completely deprotonated ("desaturated"), a dicarboxylate undergoes a two-step association reaction with increasing hydronium ion concentration.
For correct statistical analysis is it necessary to keep track of binding to the right and left sides of the molecule.
Continue the acid-base titration analysis of dicarboxylic acid by opening the Excel spreadsheet below.
Dicarboxylic Acid Titration:
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© Duane W. Sears
Revised: August 10, 1998