Hill Analysis of Interactive Ligand Binding (lig)
In this exercise, the ligand binding interactions between the bivalent ligand binding sites of symmetrical dicarboxylic acid are examined using a Hill plot. When fully protonated ("saturated"), a dicarboxylic acid undergoes a two-step dissociation reaction with decreasing hydronium ion concentration. When completely deprotonated ("desaturated"), a dicarboxylate undergoes a two-step association reaction with increasing hydronium ion concentration.
For correct statistical analysis is it necessary to keep track of binding to the right and left sides of the molecule.
Two-step dissociation | |||||||||||||||||||||||||||||||||||||||||||||||||||||
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Two-step association | |||||||||||||||||||||||||||||||||||||||||||||||||||||
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Continue the acid-base titration analysis of dicarboxylic acid by opening the Excel spreadsheet below.
Dicarboxylic Acid Titration:
Hill Plot
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© Duane W. Sears
Revised: August 10, 1998