| 34 Recombinant DNA Technology
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After reading this chapter you should be able to:
- Explain the basis of the molecular attraction that causes nucleic acid hybridization reactions to occur; explain the difference between the probe and target sequences in hybridization assays.
- Describe what is meant by a 'primer pair' used in a PCR assay, and explain how the sequence of these primers determines the product amplified in the PCR process.
- Describe the typical characteristics of restriction endonucleases with regard to their selection of cleavage sites on DNA and the types of fragment ends that are produced.
- Describe the general steps used to perform a Southern blot and the type of information it provides.
- Differentiate between a cDNA and a genomic clone.
- Describe typical features of a bacterial plasmid used for cloning DNA.
- Provide examples of investigations in which it is desirable to carry out hybridization reactions under conditions of either high or low stringency.
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