| Inhibitors of the ADP-ATP translocase
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| Figure 8.18 Oligomycin inhibition of oxygen uptake. Oligomycin inhibits oxygen uptake in ATP-synthesizing mitochondria. Oligomycin inhibits ATP synthase and oxygen uptake in coupled mitochondria. However, DNP stimulates oxygen uptake after oligomycin inhibition, by dissipating the proton gradient. |
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| Most ATP is synthesized in the mitochondrion, but used in the cytosol for biosynthetic reactions. Newly synthesized
mitochondrial ATP and spent cytosolic ADP are exchanged by a mitochondrial ADP-ATP translocase, representing about 10% of the protein in the inner mitochondrial membrane (Fig. 8.4). This translocase can be inhibited by unusual plant and mold toxins, such as bongkrekic acid and atractyloside. Their effects are similar to those of oligomycin in vitro - a proton gradient builds up and electron transport stops, but in this case respiration can be reactivated by uncouplers.
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