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Structure of Dnmt3a-Dnmt3L C-terminal domain complex
2QRV
Primary Citation

Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation.

Jia, D.,  Jurkowska, R.Z.,  Zhang, X.,  Jeltsch, A.,  Cheng, X.

Journal: (2007) Nature 449: 248-251

PubMed: 17713477  
PubMedCentral: PMC2712830  
DOI: 10.1038/nature06146  
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PubMed Abstract:
Genetic imprinting, found in flowering plants and placental mammals, uses DNA methylation to yield gene expression that is dependent on the parent of origin. DNA methyltransferase 3a (Dnmt3a) and its regulatory factor, DNA methyltransferase 3-like protein (Dnmt3L), are both required... [ Read More & Search PubMed Abstracts ]
 
  •   Molecular Description Hide
    Classification: Transferase/transferase Regulator
    Structure Weight: 244154.03
    Molecule: DNA (cytosine-5)-methyltransferase 3A
    Polymer: 1 Type: protein Length: 295
    Chains: A, D, E, H
    EC#: 2.1.1.37   
    Fragment: residues 623 to 908
    Organism Homo sapiens
    Gene Name DNMT3A
    UniProtKB:   Protein Feature View | Search PDB | Q9Y6K1  
    Q9Y6K1Molec. ProcessingDNA (cytosine-5)-methyltransferase 3AMotifPWWPADDGAPHD-Interaction witInteraE.C.2.1.1.37: DNA (cytosine-5-)-methyltransferaseUP SitesUniProtKBPDB SitesSecstruc2QRV.A2QRV.E2QRV.D2QRV.HPDBTooltip
    Molecule: DNA (cytosine-5)-methyltransferase 3-like
    Polymer: 2 Type: protein Length: 230
    Chains: B, C, F, G
    Fragment: residues 160 to 386
    Organism Homo sapiens
    Gene Name DNMT3L
    UniProtKB:   Protein Feature View | Search PDB | Q9UJW3  
    Q9UJW3Molec. ProcessingDNA (cytosine-5)-methyltransferase 3-likeMotifADDGATA-PHD-type;UniProtKBSecstruc2QRV.B2QRV.C2QRV.F2QRV.GPDBTooltip
     
  •   Source Hide
    Polymer: 1
    Scientific Name: Homo sapiens   Taxonomy   Common Name: Human Expression System: Escherichia coli bl21(de3)  
    Polymer: 2
    Scientific Name: Homo sapiens   Taxonomy   Common Name: Human Expression System: Escherichia coli bl21(de3)  
     
  •   Related PDB Entries Hide
    Identifier Details
    2PV0  Dnmt3L 
    2PVC  Dnmt3L-Histone H3 tail 
     
  •   Ligand Chemical Component Hide
    Identifier Formula Name Interactions
    SAH
    Search 
    Download      		SAH C14 H20 N6 O5 S
    S-ADENOSYL-L-HOMOCYSTEINE
    		SAH:2QRV
     
  •   External Domain Annotations Hide
     
  •   Structural Biology Knowledgebase Data Hide

    Information from the Structural Biology Knowledgebase  

     
 
Data in orange boxes are gathered from external resources (when available).
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  Biological Assembly 1       
Biological assembly 1 assigned by authors
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  •   Deposition Summary Hide
    Authors:   Jia, D.,  Cheng, X.

    Deposition:   2007-07-29
    Release:   2007-12-04
    Last Modified (REVDAT):   2009-02-24
     
  •   Revision History    Hide
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    2011-07-13
    Version format compliance
     
  •   Experimental Details Hide
    Method:   X-RAY DIFFRACTION
    Exp. Data:
      Structure Factors
    EDS  
    Resolution[Å]:   2.89
    R-Value: 0.259 (obs.)
    R-Free: 0.281
    Space Group: H 3
    Unit Cell:
      Length [Å] Angles [°]
    a = 401.88 α = 90.00 
    b = 401.88 β = 90.00 
    c = 49.69 γ = 120.00