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TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH HEMIMETHYLATED DNA AND ADOHCY
DOI:10.2210/pdb5mht/pdb   NDB ID: PDEB123
5MHT
Primary Citation

A structural basis for the preferential binding of hemimethylated DNA by HhaI DNA methyltransferase.

O'Gara, M.,  Roberts, R.J.,  Cheng, X.

Journal: (1996) J.Mol.Biol. 263: 597-606

PubMed: 8918941  
DOI: 10.1006/jmbi.1996.0601  
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PubMed Abstract:
The crystal structure of HhaI methyltransferase complexed with non-palindromic duplex DNA, containing a hemimethylated recognition sequence, and with the cofactor analog S-adenosyl-L-homocysteine (AdoHcy), has been determined. The structure provides an explanation for the stronger affinities of DNA methyltransferases for hemimethylated... [ Read More & Search PubMed Abstracts ]
 
  •   Molecular Description Hide
    Classification: Transferase/dna
    Structure Weight: 44767.78
    Molecule: DNA (5'-D(*CP*CP*AP*TP*GP*(5CM)P*GP*CP*TP*GP*AP*C)-3')
    Polymer: 1 Type: dna Length: 12
    Chains: C
    Molecule: DNA (5'-D(*GP*TP*CP*AP*GP*CP*GP*CP*AP*TP*GP*G)-3')
    Polymer: 2 Type: dna Length: 12
    Chains: D
    Molecule: PROTEIN (HHAI METHYLTRANSFERASE)
    Polymer: 3 Type: protein Length: 327
    Chains: A
    EC#: 2.1.1.37   
    Organism Haemophilus haemolyticus
    Gene Name hhaIM
    UniProtKB:   Protein Feature View | Search PDB | P05102  
    P05102Molec. ProcessingModification methylase HhaIMotifSAM-dependent MTase C5-typeE.C.2.1.1.37: DNA (cytosine-5-)-methyltransferaseUP SitesUniProtKBSCOP domainsS-adenosyl-L-methionine-dependent methyltransferases PDB SitesSecstruc5MHT.APDBTooltip
     
  •   Structure Validation Hide

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  •   Source Hide
    Polymer: 1
    Scientific Name: Synthetic construct   Taxonomy    
    Polymer: 2
    Scientific Name: Synthetic construct   Taxonomy    
    Polymer: 3
    Scientific Name: Haemophilus haemolyticus   Taxonomy    
     
  •   Ligand Chemical Component Hide
    Identifier Formula Name View Interactions
    SAH
    Search 
    Download      		SAH C14 H20 N6 O5 S
    S-ADENOSYL-L-HOMOCYSTEINE
    		SAH:5MHT
     
  •   Modified Residues Hide
    Identifier Formula Parent Type
    5CM
    Search      		5CM C10 H16 N3 O7 P DC dnaLinking
     
  •   External Domain Annotations Hide
     
  •   Structural Biology Knowledgebase Data Hide

    Information from the Structural Biology Knowledgebase  

     
  • Nucleic Acid Database Hide
    View the NDB ID associated with this structure: PDEB123
     
 
Data in orange boxes are gathered from external resources (when available).
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  Biological Assembly       
No symmetry
Stoichiometry: Monomer
Biological assembly 1 assigned by authors
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  •   Deposition Summary Hide
    Authors:   Cheng, X.

    Deposition:   1996-10-22
    Release:   1997-07-23
    Last Modified (REVDAT):   2009-02-24
     
  •   Revision History    Hide
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    2011-07-13
    Version format compliance
     
  •   Experimental Details Hide
    Method:   X-RAY DIFFRACTION
    Exp. Data:
    N/A
    Resolution[Å]:   2.70
    R-Value: 0.188 (obs.)
    R-Free: n/a
    Space Group: H 3 2
    Unit Cell:
      Length [Å] Angles [°]
    a = 99.86 α = 90.00 
    b = 99.86 β = 90.00 
    c = 325.20 γ = 120.00