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TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH ADOHCY AND DNA CONTAINING 4'-THIO-2'DEOXYCYTIDINE AT THE TARGET
DOI:10.2210/pdb6mht/pdb   NDB ID: PDE141
6MHT
Primary Citation

DNA containing 4'-thio-2'-deoxycytidine inhibits methylation by HhaI methyltransferase.

Kumar, S.,  Horton, J.R.,  Jones, G.D.,  Walker, R.T.,  Roberts, R.J.,  Cheng, X.

Journal: (1997) Nucleic Acids Res. 25: 2773-2783

PubMed: 9207024  
PubMedCentral: PMC146812  
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PubMed Abstract:
4'-Thio-2'-deoxycytidine was synthesized as a 5'- protected phosphoramidite compatible with solid phase DNA synthesis. When incorporated as the target cytosine (C*) in the GC*GC recognition sequence for the DNA methyltransferase M. HhaI, methyl transfer was strongly inhibited. In contrast, these... [ Read More & Search PubMed Abstracts ]
 
  • Nucleic Acid Database Hide
    View the NDB ID associated with this structure: PDE141
     
 
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  •   Deposition Summary Hide
    Authors:   Cheng, X.

    Deposition:   1998-08-05
    Release:   1998-08-12
    Last Modified (REVDAT):   2009-02-24
     
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    2011-07-13
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  •   Experimental Details Hide
    Method:   X-RAY DIFFRACTION
    Exp. Data:
    N/A
    Resolution[Å]:   2.05
    R-Value: 0.186 (work)
    R-Free: 0.231
    Space Group: H 3 2
    Unit Cell:
      Length [Å] Angles [°]
    a = 99.86 α = 90.00 
    b = 99.86 β = 90.00 
    c = 325.20 γ = 120.00