Instructor: Dr. Natalia Tretyakova, Ph.D. «hyperlink
"mailto:Trety001@umn.edu"»
- 6-3432
PDB reference correction and design Dr.chem., Ph.D. Aris
Kaksis, Associate Prof.
mailto:ariska@latnet.lv
DNA
sequence specific binding Proteins 3
Catabolite
Gene Activator Protein Act1 with DNA 30 bp fragment
1CGP.PDB
---> C chain
5'-GCGAA AA GTGTGAC A T A T |GT C ACAC
TTTTCG-3' chain D --->
<--- C chain
3'-GCTT TTCACACTG| T A T A CA G TGTG AAAA GCG-5' chain D <---
Binding TATA Box Protein on DNA 16 base pair specific
Site
fragment
1YTF.PDB
<--- F chain
3'-ACA T ACA T A T A TTTTG-5' chain F <---
---> E chain
5'-TGT A TGT A T A T AAAAC-3'
chain E --->
U
turn binding protein Ihf with DNA 35 = 20+15 base paire fragment
1IHF.PDB
<--- C35
chain 3'-CGGTGC AAC AAA TT GAAA GC AATGC
TTTTTT GGC-5' chain C35
<---
---> E20
chain 5'-CCACG TTGTTT AA CTATT CG|TTACG AA AA AA CCGG-3' chain D15 --->
Human Protein SRY binding DNA 8 base
pair
fragment
1HRY.PDB
<--- B chain
3'-GTTTGTGC-5' chain B
<---
---> B chain
5'-C AAACACG-3'
chain B --->
Estrogen
O Receptor on 18 base paire DNA box fragment
1HCQ.PDB
---> C chain
5'-CC A GG T C A
C A G T G A CC
T G-3' chain C --->
<--- D
chain 3'-G
T CC A G T G T
C A C
T GG A CC-5' chain D
<---
RiboNuclease with deoxy
AAAA
ssDNA chaine fragment
1RBJ.PDB
Restriction
EndoNuclease bamH with 12 base paire DNA specific site
1BHN.PDB
---> C chain
5'-
T A
T GG A T
CC A T A-3' chain C --->
<---
D chain 3'-
T A CC
T A GG T A T
Restriction EndoNuclease Eco RvRI on 12
base
pair DNA specific site
1RVA.PDB
---> C chain
5'-AA A G A T A T
C T T
-3' chain C --->
<--- D chain 3'-
T T
C T A T A G A AA
Restriction
EndoNuclease PvuII with 13 base paire DNA specific site
1PVI.PDB
---> C chain
5'-
T G A CC A GC
T GG
T C-3'
chain C --->
<--- D chain
3'-C T GG T
CG A CC A
G
T