Mutations in GroEL - Page 1

Residues 1 - 237

NOTE: Residue numbering includes N-terminal Met. Mutations are listed in sequence order (1-548).

Mutation

Effect

Reference

A3SIncreased cooperativity of ATP hydrolysis
Decreased GroEL stability

(5)

K4EDissociation of oligomer

(6)

K4IDecreased GroEL stability

(6)

K4RDecreased GroEL stability

(6)

R13GDecrease in ATPase activity

(1)

R13G/A126VDecrease in ATPase activity

(1)

D25RDecrease in OTC refolding; Small colonies of LG6 strain

(1)

F44ADecrease in ATPase activity; No growth of LG6 strain

(1)

G45EDecrease in OTC refolding; Small colonies of LG6 strain

(1)

S55ENo effect detected

(1)

K65ENo effect detected

(1)

E76KNo effect detected

(1)

D87KDecreased GroES binding; Decreased OTC folding (no release)
No growth of LG6 strain

(1)

D87EDecreased ATP hydrolysis; Decreased polypeptide binding
Decreased Rubisco refolding

(4)

D87NDecreased GroES binding; Decreased OTC folding (no release)
No growth of LG6 strain

(1)

A96ENo effect detected

(1)

V107ENo effect detected

(1)

I150ENo ATPase, No GroES binding, No OTC refolding (no release)
No growth of LG6 strain

(1)

S151VNo ATPase, No GroES binding, No OTC refolding (no release)
No growth of LG6 strain

(1)

A152ENo ATPase, No GroES binding, No OTC refolding (no release)
Tiny colonies in LG6 strain

(1)

D196ANo effect detected

(1)

R197ANo effect detected

(1)

R197ADecreased positive cooperativity in ATP hydrolysis
Strong substrate (ATP) inhibition

(2)

Y199ENo GroES binding, No OTC binding, No growth of LG6 strain

(1)

L200ENo effect detected

(1)

S201EDecreased OTC binding, Decreased OTC refolding
No growth of LG6 strain

(1)

S201FDecreased ATPase activity, Decreased GroES binding
Decrease in GroEL stability

(3)

Y203ENo GroES binding, No OTC binding, No growth of LG6 strain

(1)

F204ENo GroES binding, No OTC binding, Tiny colonies in LG6 strain

(1)

K225ESmall colonies in LG6 strain

(1)

I227SNo effect detected

(1)

I230SNo effect detected

(1)

L234EDecreased ATPase, No GroES binding
No OTC binding, No growth of LG6 strain

(1)

L237ENo GroES binding, No OTC binding, No growth of LG6 strain

(1)

This information is accurate to the best of my knowledge. However, you should check the references cited for a more complete understanding of these mutations and their effects.

References

  1. Fenton et al (1994) Nature371:614-619.
  2. Yifrach et al (1994) J. Mol. Biol. 243:397-401.
  3. Baneyx & Gatenby (1992) J. Biol. Chem. 267:11637-11644.
  4. Weiss & Goloubinoff (1995) J. Biol. Chem. 270:13956-13960.
  5. Horovitz et al (1993) J. Mol. Biol. 231:58-64.
  6. Horovitz et al (1993) J. Biol. Chem. 268:9957-9959.

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