Structural Features of the Chaperonins

Structures of GroES/Hsp10

A Kinemage Tour of the GroES Structure - Courtesy of Sam Landry

Updated 12/01/97 to include the mobile loop from the crystal structure, and the trNOE structure of the mobile loop superimposed upon it.

To view the kinemage tour of GroES you need to download the Mage software from the following FTP sites:

MacIntosh: Here
PC: Here

Note: if using a MACINTOSH, you must use Mage version 4.0 in order to view this kinemage. Thanks to Will McClure for pointing this out.

You can configure your Web browser to associate the *.kin extension with the Mage viewer, or you can save the kinemage file to disk and view at your leisure. For configuring your browser to read kinemage files, the MIME type is chemical and the subtype is x-kinemage. The file extension is kin.

NMR structure of GroES mobile loop peptide bound to GroEL from Sam Landry

Crystal structure of Hsp 10 from Mycobacterium leprae (84 Kb)

X-ray Crystal Structures of GroEL

The structure of a GroEL monomer taken from the original structure determination (335 Kb)
The refined x-ray structure of a GroEL heptamer (2.25 Mb)
The x-ray structure of a GroEL 14mer complexed with ATP-gamma-S (5.8 Mb)
The x-ray structure of a GroEL14-ADP7-GroES7 asymmetric complex (4.99 Mb)
The x-ray structure of a GroEL apical domain fragment (110 kb)
The x-ray structure of a GroEL apical domain fragment double mutant A262L, I267M (162 kb)

WARNING: These PDB files are large. Viewing them through your Web browser may be VERY slow. In order to best view these files, you might want to save them to your disk and view them from your hard drive.

To view PDB files, download free Rasmol software here
An Overview of the GroEL and GroES Structures can be found here

This page was created by Will McClure as part of the Physical Biochemistry Course at Carnegie Melon. There are nice pictures of GroEL highlighting residues involved in GroES binding, polypeptide binding and ATP binding.

Gif files of the Cpn10 from Mycobacterium leprae courtesy of Christophe Verlinde.

For a reference, see: Mande, et al. Science 271: 203-207 (1996)

A space filling model of the Cpn10 heptamer .
An electrostatic potential surface viewed from inside the heptamer highlighting the negative charge around the roof.

Electron Microscopy Images and Movies of the GroEL ATPase Cycle

Cryo-EM Images from Helen Saibil's Chaperone Page at Birkbeck
Movies of the ATPase cycle from Birkbeck.
Images from Jose Valpuesta's Group in Spain
Images of symmetric GroEL-GroES complexes with two rhodanese bound from Jose Valpuesta's Group in Spain

Structures of other Molecular Chaperones

Back to the Chaperonin Page
Email comments or suggestions to Jeff Seale
Last Updated December 26, 1997