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HYBRIDIZATION
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Hybridization is based on the annealing properties of DNA
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Double-stranded DNA exists in life as a double helix, in which the two strands are held together by the hydrogen bonds between complementary bases (A with T, and G with C).
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Hybridization is a fundamental feature of DNA technology. It is a process by which a piece of DNA or RNA of known nucleotide sequence, which can range in size from as little as 15 bp to several hundred kilobases, is used to identify a region or fragment of DNA containing complementary sequences. The first piece of DNA or RNA is called a probe. Probe DNA will form complementary base pairings with another strand of DNA, often termed the target, if the two strands are complementary, and a sufficient number of hydrogen bonds is formed.
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