| DNA AMPLIFICATION AND CLONING
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| Two methods exist for mass amplification of DNA in vitro
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| The amplification of DNA is central to the study of molecular biology and genetics. DNA amplification makes possible an enormous increase in the number of copies of a desired DNA sequence. Two important approaches to the amplification of DNA are:
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- cell-based DNA cloning: DNA is amplified in vivo by a cellular host such that the number of copies of the desired DNA template increases simply due to the exponential increase in number of replicating host cells;
- enzyme-based DNA cloning (cell-free): this method is represented by the polymerase chain reaction (PCR) and involves entirely in vitro DNA amplification.
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